A mouse fibroblast cell line was incubated with propidium iodide (PI, red), infected with GFP-tagged HSV-1, and then monitored by time-lapse fluorescence microscopy. After HSV-1 infection, the GFP intensity increased before plasma membrane rupture, indicating that viral protein accumulation is involved in cell death. For additional details, see Huang et al. and related paper by Guo et al.

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